Fig. 6
Genipin exhibits anti-HCC effects in PDX mice model. (a) Protein expression of STAT-3 and p-STAT-3 (Y705) in human HCC tissue and para-carcinoma tissue were examined by western blot assay. (b) Graphical representation of constructing PDX mice model. Protein expression of STAT-3 and p-STAT-3 (Y705) were detected in F1, F2, and F3 generation. (c) Tumor sizes were measured after genipin treatment in PDX model (n = 8). (d) Tumor volume in PDX mice was examined after genipin treatment (n = 8). (e) Tumor weight in PDX mice was examined after genipin treatment (n = 8). (f) Expression of p-STAT-3, STAT-3, Survivin, Bcl-2, and VEGF was examined by western blotting in HCC tissues. (g) Expression of CD31 and p-STAT-3 was validated by immunohistochemistry assay in HCC tissues. Scale bar 40 μm. Data presented as mean ± SD. **P < 0.01 and ***P < 0.001