Fig. 5

SMURF1 facilitated the ubiquitination of PTEN protein and promoted cell proliferation in DU145 and LNCap cells. a-b. The mRNA and protein levels of SMURF1 were determined by RT-PCR and western blotting assays following cell infection with OE-SMURF1, OE-NC, sh-SMURF1 or sh-NC. c The ubiquitination of PTEN protein was assessed by IP assay after 48 h of cell infection with OE-SMURF1, OE-NC, sh-SMURF1 or sh-NC. d-e The effects of SMURF1 up−/downregulation on cell proliferation were assessed by CCK-8 assay in DU145 and LNCap cells. f The effects of SMURF1 up−/downregulation on DU145 and LNCap cell apoptosis was detected by flow cytometry assay (Cells in Q2 and Q3 were considered as the apoptotic cells). (^**p < 0.01, ^***p < 0.001, sh-SMURF1 group compared with sh-NC group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, OE-SMURF1 group compared with OE-NC group)