Fig. 6

IMP3 facilitated prostate cancer cell proliferation and tumorigenesis through upregulating SMURF1 expression. DU145 and LNCap cells in OE-IMP3, OE-IMP3 + sh-SMURF1 and control (OE-NC + sh-NC) groups were collected for the following assays. a IP assay was used to assess the crosstalk between Ub and PTEN proteins. b-c CCK-8 was applied to determine cell proliferation. d-e Flow cytometry was applied to detect cell apoptosis (Cells in Q2 and Q3 were considered as the apoptotic cells). f In vivo tumor formation assay was performed to detect the effect of IMP3/SMURF1 axis on the tumorigenesis of DU145 and LNCap cells. (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, OE-IMP3 group compared with control group; ^#p < 0.05, ^##p < 0.01, OE-IMP3 + sh-SMURF1 group compared with OE-IMP3 group)