Fig. 2

NEK2 silencing suppresses the growth and proliferation and induces the apoptosis of cervical cancer cells in vitro. a HeLa and SiHa cells were transfected with indicated siRNAs for 48 h, and samples were harvested and analyzed by Western blotting with the indicated antibodies (n = 3). b NEK2 silencing inhibited the growth of HeLa and SiHa cells (n = 3). c Colony formation ability was significantly reduced in NEK2-depleted cells. *** P < 0.001 (n = 3). d Knockdown of NEK2 reduced the proliferation of HeLa and SiHa cells, as determined by an EdU cell proliferation assay. ** P < 0.01, *** P < 0.001 (n = 3). Scale bar: 200 μm. e HeLa and SiHa cells transfected with indicated siRNAs for 48 h were subjected to annexin V-EGFP/propidium iodide staining, and analyzed by flow cytometry. *** P < 0.001 (n = 3)