Fig. 3

Downregulation of NEK2 inhibited the oncogenic behavior of cervical cancer cells in vitro and in vivo. a Western blotting analyses showed that NEK2 was efficiently knocked down in SiHa cells by ShRNA transfection. b Stable knockdown of NEK2 suppressed SiHa cell growth in vitro. *** P < 0.001 (n = 3). c Colony formation ability was reduced in the ShNEK2 groups compared with the ShControl group. *** P < 0.001 (n = 3). d Representative images of the xenograft tumors in three groups (n = 7 mice/group). e The growth curves of the xenograft tumors in three groups were presented. Tumor volumes were calculated every three or four days. Data was shown as the mean tumor volume ± SEM (n = 7 mice/group). *** P < 0.001. f The tumor weights in three groups were presented. * P < 0.05, ** P < 0.01