Fig. 6

ZFPM2-AS1 regulates the expression of the miR-139 target gene GDF10. a-b Bioinformatics analysis predicted that GDF10 has a negative correlation with miR-139. The predicted miR-139 binding site in GDF10 were also displayed. c Luciferase reporter assay for the detection of pMIR-GDF10 /3′-UTR-WT and pMIR GDF10/3’-UTR-Mutluciferase activity cotransfection with miR-139, miR-NC, miR-139 + pcDNA-NC or miR-139 + pcDNA/ZFPM2-AS1 in Huh7 cells. d and e The expression of GDF10 mRNA and protein was detected by PCR (d) and western blotting (e) after transfection of Huh7 and HCCLM3 cells. GAPDH and β-actin were used as internal controls. The data are expressed as the mean ± standard deviation, **P < 0.01