Fig. 2

Glucose and HbA1c levels, body weight and hyperglycaemia-associated carbonyl stress. Blood glucose levels and body weight during the study period (a and b) and at the end of the study period (22 weeks of age¹) (c and d) and HbA1c levels (e), and serum levels of AGEs (f) and total PCOs (g) at the end of the study period (22 weeks of age¹) in control (Ctr), Ctr treated with FL-926-16 (Ctr + FL), diabetic (Diab), and Diab treated with FL-926-16 (Diab+FL) KCM mice. Statistical significance between groups for time course of blood glucose (a) and body weight (c) was calculated using two-way ANOVA followed by the Bonferroni post-test. Each time point represents mean ± SD of 12 animals until the 17th week of age, and 9–12 animals from the 18th to the 22nd week of age. Statistical significance for blood glucose (c), body weight (d), serum levels of AGEs (e) and PCOs (f) at 22 weeks of age¹ was assessed using one-way ANOVA followed by the Student-Newman-Keuls test for multiple comparisons. Each dot represents one case and bars represent mean ± SEM. ***P < 0.001 or *P < 0.05 vs Ctr; †††P < 0.001 vs Diab. ¹Except for three Diab and one Diab + FL mice, which were killed 5 and 3 weeks, respectively, before the end of the study (see “Results” section for further details).