Fig. 6

Differential mechanisms underlying RCS- and AGE-induced YAP activation. YAP protein level (a) by Western blot in MIA PaCa-2 siNT control and siYAP#1 and #2 cells after 48 h. CTGF, WNT5a and EMP2 mRNA levels (b) in MIA PaCa-2 siNT control and siYAP#2 cells exposed to MGO (200 μM) or CML (100 μg/mL); n = 5 wells in duplicate per condition. LATS1 (c) and total and phosphorylated EGFR (d) levels in MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h and relative band densitometry analysis from three separate experiments. Analysis of EGFR silencing by Western blot (e) in MIA PaCa-2 cells after 48 h. Western blot analysis for YAP (f) in nuclear extracts from MIA PaCa-2 siNT control and siEGFR cells exposed to CML (100 μg/mL) for 48 h and relative band densitometry analysis from three separate experiments. Each dot represents a single experiment and bars represent mean ± SD. Post hoc multiple comparison: ***P < 0.001, **P < 0.01 or *P < 0.05 vs Ctr; †††P < 0.001 or ††P < 0.01 vs treated (MGO or CML) siNT control. siNT = non-target control