Fig. 5

The miR-216b/JMJD2C/HIF1α/HES1 signaling axis is involved in cisplatin-induced apoptosis in OS cells. a Transfection efficiency of miR-216b and HES1 expressions in MG63 and SaOS-2 cells was determined by RT-qPCR and Western blot analysis, relative to U6 and β-actin, respectively. b Cell viability of MG63 and SaOS-2 cells was determined by CCK-8 assay. c Apoptosis of MG63 and SaOS-2 cells was determined by flow cytometry. * p < 0.05 vs. cells treated with agomir NC + oe-NC; # p < 0.05 vs. cells treated with miR-216b agomir + oe-NC. Data in panel (a) and (c) were compared by one-way ANOVA with Tukey’s test, while data in panel (b) were compared by repeated measures ANOVA with Bonferroni test. Data are shown as mean ± standard deviation of three technical replicates