Fig. 2

RND2 knockdown induced GBM cell apoptosis in vitro. a-b Effects of RND2 knockdown on cleaved Caspase-3 expression in U87 cells according to immunofluorescence. Scale bars, 100 μm. **P < 0.01. c-d Effects of RND2 knockdown on the ΔΨm in U87 cells according to JC-1 staining. A decrease in the ratio of red (aggregates)/green (monomers) fluorescence intensity indicates the loss of ΔΨm. Scale bars, 50 μm. **P < 0.01. e-f U87 cell death was detected by TUNEL staining. Scale bars, 50 μm. Statistical analysis of the positive rate is showed. **P < 0.01. g-i U87 cells were transfected with negative control shRNA (shNC) or shRNAs against RND2 (shRND2–1 and shRND2–2), followed by Annexin V-PE/7-AAD staining and flow cytometric analysis. Cell apoptosis was calculated by FACS. **P < 0.01. j-k Effects of RND2 overexpression and knockdown on the levels of apoptosis-related proteins in GBM cells. shNC: negative control shRNA; shRND2–1 and shRND2–2: two shRNAs against RND2; Flag: the control group; Flag-RND2: RND2 overexpression group. All bar plot data are the means ± SD. The data and graphs are representative of three independent experiments with similar results