Fig. 3

RND2 overexpression reduces GBM cell autophagy in vitro. a-b Representatives images of IHC staining showed RND2 expression inversely correlates with LC3B in the same location but positively correlates with p62. Scale bars, 50 μm. c Effects of RND2 overexpression on LC3B expression in U87 cells according to immunofluorescence. Scale bars, 100 μm. **P < 0.01. d The numbers of GFP-LC3 puncta were quantified using confocal laser scanning microscopy in U87-MG cells transfected with GFP-LC3. Scale bars, 10 μm. **P < 0.01. e Transfected cells were prepared for transmission electron microscopy analysis. The number of autophagic vacuoles was analysed and the red arrows indicate autophagic vacuoles. f Effects of RND2 overexpression on the levels of autophagy-related proteins in GBM cells. g Effects of RND2 knockdown on the levels of autophagy-related proteins in GBM cells. **P < 0.01. shNC: negative control shRNA; shRND2–1 and shRND2–2: two shRNAs against RND2; Flag: the control group; Flag-RND2: RND2 overexpression group. All bar plot data are the means ± SD. The data and graphs are representative of three independent experiments with similar results