Fig. 5

RND2 physically interacts with p38 and inhibits p38 phosphorylation in vitro and in vivo. a Immunofluorescent staining (IF) analyses of the co-localization of exogenous RND2 and p38 in U87 cells, Scale bar 10 μm. b Co-immunoprecipitation assays in U87 cells determined the physical interaction between RND2 and p38. c Effects of RND2 overexpression and knockdown on the protein levels of p-p38 and p38 in U87 cells. d IHC analyses of RND2, p-p38 and p38 in mouse tumour sections. shNC: negative control shRNA; shRND2–1 and shRND2–2: two shRNAs against RND2; pcDNA3.1: the control group; Flag-RND2: RND2 overexpression group. All bar plot data are the means ± SD. The data and graphs are representative of three independent experiments with similar results