Table 1 Primer list
Primers for qPCR | |
|---|---|
Genes | Primer sequences (5′--3′) |
 Primers U1 -F | GGGAGATACCATGATCACGAAGGT |
 Primers U1 -R | CCACAAATTATGCAGTCGAGTTTCCC |
 JAK2-F | ATCCACCCAACCATGTCTTCC |
 JAK2-R | ATTCCATGCCGATAGGCTCTG |
 JAK3-F | CCTGATCGTGGTCCAGAGAG |
 JAK3-R | GCAGGGATCTTGTGAAATGTCAT |
 STAT1-F | CAGCTTGACTCAAAATTCCTGGA |
 STAT1-R | TGAAGATTACGCTTGCTTTTCCT |
 STAT3-F | ATCACGCCTTCTACAGACTGC |
 STAT3-R | CATCCTGGAGATTCTCTACCACT |
 SOCS1-F | TTTTCGCCCTTAGCGTGAAGA |
 SOCS1-R | GAGGCAGTCGAAGCTCTCG |
 LINC00669-F | CAGTGAGATGCAGAGCTTGG |
 LINC00669-R | TGTCCTTGAGGCTGTCTGTG |
 β-actin -F | CATGTACGTTGCTATCCAGGC |
 β-actin -R | CTCCTTAATGTCACGCACGAT |
Oligonucleotide sequence for RNA pulldown | |
 LINC00669-sense-F | TAATACGACTCACTATAGGGAGACTGTGTGGAACTTGGGATTCGAACGG |
 LINC00669-sense-R | CAATTTTGATTGGCTTTATTTTGATGTG |
 LINC00669-antisense-F | CTGTGTGGAACTTGGGATTCGAACGG |
 LINC00669-antisense-R | TAATACGACTCACTATAGGGAGACAATTTTGATTGGCTTTATTTTGATGTG |