Fig. 2

Effects of EIF3H on ESCC cell growth in vitro and in vivo. a-d EIF3H was stably overexpressed or knocked down in KYSE150 and KYSE510 cells. The immunoblotting showed the protein level of Snail and EIF3H (a and c), and the RT-qPCR assay was performed to detect the mRNA level of Snail and EIF3H (b and d). e-h CCK-8 or colony formation assay was performed in these established cells. Overexpression of EIF3H promoted cell proliferation and colony formation (E and F). Knockdown of EIF3H inhibited cell proliferation and colony formation. Data are shown from three independent experiments (g and h). i EIF3H knockdown suppressed xenograft tumor growth in vivo in mice. KYSE150 cells with or without knockdown of EIF3H were inoculated subcutaneously into the flanks of BALB/c nude mice. Tumor size was monitored for the indicated timeframe. Tumor weight was measured after the experiment. j IHC for Ki67 of representative areas of shControl, shEIF3H#1, and shEIF3H#2 group (left panel). Scale bars, 200 μm. Quantification of Ki67 positive cells in representative areas of shControl, shEIF3H#1, and shEIF3H#2 group (right panel). *P < 0.05; **P < 0.01; ***P < 0.001