Fig. 3

Effect of hsa_circ_0002577 on EC cell migration/invasion. HEC-1-B and Ishikawa cells were transfected with designated vectors or sequences. a The migration capability of cells after transfection was evaluated by wound healing assay. The relative migration rate was analyzed. b Transwell assay was performed at 48 h post-transfection and the number of migrated and invaded cells were counted. c Cell supernatants were collected from transfected HEC-1-B and Ishikawa cells. HUVECs were cultured with a mixture of EC cell supernatants and ECGM for 4 days. Then Transwell assay was performed and the migration rate was calculated. d Cell supernatants were collected from transfected HEC-1-B and Ishikawa cells. HUVECs were cultured with a mixture of EC cell supernatants and ECGM for 4 days. HUVECs treated with different cell supernatants were transferred to Matrigel-coated wells and incubated at 37 °C for 24 h. The number of branches in each group of cells was quantified