Fig. 2

Exosomal miR-141 derived from SCLC cells promotes HUVEC migration and tube formation. A, B. After miR-141-mimic transfection, the relative expression of miR-141 was increased in H446 and H1048 cells and in their exosomes. C. The size and quantitation of exosomes isolated from miR-141- or NC-transfected H446 cells was examined by nanoparticle tracking analysis. D. TEM images of exosomes isolated from H446 cells. E. The level of CD9 and TSG101 was detected by western blot. F. Representative images of HUVECs that migrated through transwell inserts after incubation with H446 cell- or H1048 cell-derived exosomes, with the numbers of migrated cells indicated in the chart to the right. G. Representative images of tubes formed by HUVECs after incubation with H446 cell- or H1048 cell-derived exosomes; the number of tubes formed is shown in the chart to the right. H. Representative images of mouse aortic rings from which microvessels sprouted after treatment with H446 cell- or H1048 cell-derived exosomes, with the number of sprouted microvessels indicated in the chart to the right. NC, negative control; Exo, exosomes; EXO, exosomes