Fig. 3

Depletion of KIAA1522 in lung adenocarcinoma cells reduces tumor growth and resistance to cisplatin. a Murine cell line 889 with the sgRNA-mediated depletion of KIAA1522 were injected into C57BL/6 J mice subcutaneously. One month later, the mice were sacrificed and the tumor weights were quantified. t-test, **P < 0.01. b Human adenocarcinoma cell A549 expressing KIAA1522-shRNA were injected into nude mice subcutaneously. After two months, the mice were subjected to tumor weights quantification. t-test, **P < 0.01. c Immunohistochemical staining of Ki67 in control sgRNA/Cas9 or kiaa1522-sgRNA/Cas9 expressing 889 cells. Bar = 100 μm. d IC50 of cisplatin in both 889 cells and A549 cells with down-regulated KIAA1522 were analyzed by CCK8 assays. e-f The inhibitory effects of cisplatin on 889 cells expressing kiaa1522-sgRNA/Cas9 (e) or A549 cells overexpressing KIAA1522 (f) by colony formation assays