Fig. 7

STAT1 transcriptionally regulated PD-L1 to inactivate CD8+ T cells. a-b qPCR and western blot assay indicating that PD-L1 was downregulated in the STAT1 knockdown PANC-1 and Mia paca2 cells. c-d Bioinformation analysis of the promoter binding sites of STAT1. e CHIP assay was performed to analysis the binding of STAT1 and PD-L1 promoter. f Western blot analysis for the expression level of the PD-L1 and STAT1 in PSMB8-AS1 overexpressed, STAT1 and PD-L1 downregulation PC cells. g Flow cytometric analysis of the apoptosis of CD8+ T cells in PSMB8-AS1 overexpressed and, STAT1 and PD-L1 downregulation PC cells. h Flow cytometric analysis of the activity of CD8+ T cells in PSMB8-AS1 overexpressed and, STAT1 and PD-L1 downregulation PC cells