Fig. 1

Exosomal TRIM59 is characteristically secreted by lung cancer cells and transferred to and internalized by macrophages via exosomes. a. Exosomes released by H1299 and A549 cells were detected by electron microscopy. Scale bar, 100 nm. Immunoblotting assay of indicated proteins in exosomes from H1299 and A549. b. Western blot evaluations were used to evaluateTRIM59 expression in both LC cells (H1299 and A549) exosomes and LC cells treated with TRIM59 shRNA or scrambled controls. c-d. Representative immunofluorescence image shows the internalization of PKH67-labeled H1299-derived exosomes (green) by macrophages. Confocal imaging showed the delivery of PKH67-labeled exosomes (green) to macrophages. Green dots represented delivered exosomes. Scale bar, 150 μm. e-f. THP-1 macrophages cells were incubated with exosomes for the noted periods of time or the noted doses. Western blot evaluations were used to evaluate TRIM59