Fig. 5

TRIM59 induces the macrophages to promote the proliferation, migration, and invasion of lung cancer cells. a. A schematic diagram illustrating the experimental design. THP-1 macrophages infected with lentiviral vectors expressing TRIM59 or GFP control primed with LPS for 8 h, and followed by stimulation with ATP. The effect of the conditioned medium of THP-1 macrophages on the proliferation and migration of lung cancer cells (H1299 and A549) was analyzed by CCK8, EdU analysis, and wound-healing assays. b. The effect of conditioned medium of macrophages infected with TRIM59 lentiviral particles on H1299 and A549 cells proliferation were measured by CCK8 assay. c. The effect of conditioned medium of macrophages infected with TRIM59 lentiviral particles on H1299 and A549 cells proliferation were measured by EdU analysis. Scale bars, 100 μm. d. Migration of lung cells educated by different the conditioned medium was assessed by wound healing assays. Representative results are shown, wound closure evaluated at 0 and 24 h after wounding. Quantification of wound healing percentage were performed with the Image J software. Scale bars, 200 μm. e. Invasion capacity of lung cancer cells cocultured with macrophages infected with TRIM59 lentiviral particles or GFP lentiviral control primed with LPS for 8 h, and followed by stimulation with ATP was determined by the in vitro transwell coculture system. Representative images were shown. Scale bars, 150 μm. All these experiments have been repeated three times. Data are presented as mean ± SEM. The significance was determined by Student’s t-test. ** p < 0.01