Fig. 5

Nicotine stimulates YAP1 nuclear location through association with HIF1A expression. a-b, qRT-PCR and western blot assays of HIF1A mRNA (a) and protein (b) expression levels in Panc-1 and BxPC-3 cells after treatment with different concentrations (0, 0.1,1.0 μM; 24 h) of nicotine. c-d, Western blot assays of YAP1, p-YAP1 expression levels in Panc-1 and BxPC-3 cells after transfection with the indicated vectors upon nicotine treatment. e-h, qRT-PCR assays of YAP1 target gene, CTGF, CDX2, CYR61, and CDC20, expression levels in Panc-1 (e-f) and BxPC-3 (g-h) cells after transfection with the indicated vectors upon nicotine treatment. i, Representative images of HIF1A expression and its association with YAP cellular localization in PDAC specimens based on IHC assay. Scale bars, 200 μm. *p < 0.05, **p < 0.01, ***p < 0.001