Fig. 2

TM4SF1 deficiency suppresses the migration and invasion of CRC cells. a WB and qRT-PCR analysis of the efficiency of sh-TM4SF1 and sh-Control (NC) transfection in SW480 and LoVo cells. b Wound healing assays of cell migration in SW480 and LoVo cells. The images of wound closure are presented at the indicated number of hours after scratching (0, 24 h). c Transwell assays were performed to examine the potential migration and invasion of TM4SF1 KD cells or negative control cells. d WB and qRT-PCR analysis of the expression of EMT markers in CRC cells transfected with sh-TM4SF1. e Immunofluorescence staining showed the changes in the expression of EMT-associated genes vimentin and ZO1 (red) in SW480 cells. Nuclei were counterstained with DAPI (blue). f Morphological change of SW480 cells transfected with sh-TM4SF1 and NC. *P < 0.05