Fig. 3
From: TM4SF1 promotes EMT and cancer stemness via the Wnt/β-catenin/SOX2 pathway in colorectal cancer
TM4SF1 is involved in TGF-β1-induced EMT, and modulates the stemness properties. a Wound healing assays showed that TGF-β1 enhanced the migration potential. TM4SF1 silencing decreased the migration of TGF-β1-treated SW480 cells. b Transwell assays showed that TGF-β1 enhanced the migration and invasion potential. TM4SF1 silencing suppressed the migration and invasion of TGF-β1-treated SW480 cells. c WB analysis showed that TGF-β1 increased the expression of Smad2, and EMT markers (vimentin, N-cadherin, MMP9). d TM4SF1 knockdown downregulated the expression of EMT markers in TGF-β1-treated LoVo cells. e Tumoursphere assay of CRC cells transfected with sh-TM4SF1 and NC. f CCK-8 assay showed that CRC cells with sh-TM4SF1 showed suppressed survival after treatment with fluorouracil. g. WB analysis of stemness markers (SOX2, CD44, CD133, ALDHA1) in CRC cells transfected with sh-TM4SF1. *P < 0.05