Fig. 1

ISL2 is overexpressed in gliomas and is correlated with poor patient survival. a b, c The mRNA expressions of ISL2 were shown according to WHO grades (a), glioblastoma (GBM) and lower grade glioma (LGG) (b), and isocitrate de-hydrogenase (IDH) status (c) in the CGGA database. d, e, f, g The prognostic significance of ISL2 in total grades (d), grade II (e), grade III (f), and grade IV (g) glioma tissues were detected in the CGGA database. h, i, j ISL2 was expressed at higher levels in different grade glioma tissues, compared with normal brain tissue as measured by qPCR (h), western blots (i), and immunohistochemistry (j) (grade II, n = 20; grade III, n = 25; grade IV, n = 25; NBT, n = 10). Scale bar = 50 μm. k, l, m Kaplan-Meier analysis showed the prognostic significance of the 70 glioma patients, 45 LGG patients, and 25 GBM patients with high versus low ISL2 expressions detected by qPCR. All data are expressed as the mean ± SD (three independent experiments). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001