Fig. 2
From: Autophagic flux inhibition enhances cytotoxicity of the receptor tyrosine kinase inhibitor ponatinib
PON promotes autophagy vesicle accumulation in human neuroblastoma cells. a Tumor cells were treated with vehicle (CTRL) or PON at the indicated concentrations for 24 h. The presence of cytosolic puncta was detected through immunofluorescence analyses with LC3 antibody (green). Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. b Transmission electron microscopy images present the autophagic vacuoles (AV) in SK-N-BE(2), SH-SY5Y, and IMR-32 cells after 24 h of treatment with vehicle (CTRL) or PON. The scale bar is indicated in each image. Histograms on the right show the mean number of AVs formed per cell. **p < 0.01