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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: NF-κB maintains the stemness of colon cancer cells by downregulating miR-195-5p/497–5p and upregulating MCM2

Fig. 3

Inhibition of miR-195-5p/497–5p by NF-κB activation could promote viability and inhibit apoptosis of CCSCs. a, After simultaneous overexpression of p65 and miR-195-5p/497–5p in CCSCs, the expression of p65, miR-195-5p and miR-497-5p in CCSCs was detected by RT-qPCR. b, Cell viability as detected by CCK-8 assay. c, Quantitation for cell apoptosis as detected by flow cytometry. d, The protein expression of apoptosis-related proteins (Bcl-2 and Bax) detected by Western blot analysis. Measurement data were expressed as mean ± s.d. Data among multiple groups were compared using one-way analysis of variance and then analyzed with Tukey’s post-hoc test. Cell experiment was repeated three times. * p < 0.05 vs. si-NC. # p < 0.05 vs. pcDNA-p65 + miR-NC

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