Fig. 9

miR-195-5p/497–5p could suppress the tumorigenesis and stem-like properties of CCSCs in vivo by targeting MCM2. CCSCs (SW620 CSCs and LoVo CSCs) with overexpressed miR-195-5p/497–5p and/or MCM2 were subcutaneously injected into nude mice to establish a subcutaneous xenograft tumor model. a & b, The volume and weight of xenografted tumors were observed and recorded. c, The expression of MCM2, miR-195-5p and miR-497-5p detected by RT-qPCR. d, The protein expression of MCM2, CD133, and EpCAM in tumor tissues as detected by Western blot analysis. e, The protein expression of apoptosis-related proteins (Bcl-2 and Bax) in tumor tissues as detected by Western blot analysis. Measurement data were expressed as mean ± s.d. Data among multiple groups were compared using one-way analysis of variance and then analyzed with Tukey’s post-hoc test. Repeated measures analysis of variance was used for comparing data among multiple groups at different time points, followed by Bonferroni post-hoc test. Cell experiment was repeated three times. * p < 0.05 vs. miR-NC. # p < 0.05 vs. miR-195-5p/497–5p agomir + pcDNA-3.1