Fig. 3

MiR-103a-3p knockdown suppresses the growth, proliferation, angiogenesis, and glycolysis of CRC in vivo. Nude mice were subcutaneous injected with HCT116 cells transfected with antagomir control and miR-103a-3p antagomir. a MiR-103a-3p knockdown inhibited tumour growth. Tumour volume was measured every 3 d using the formula: volume = (length × width²)/2. b The tumours weight was measured after dissecting from the nude mice at 37 d after injection. c Representative image of xenograft tumours was shown. d The level of miR-103a-3p, HK2, LDHA, and PFK1 were determined in tumour tissues from miR-103a-3p antagomir group and control group by qRT-PCR. e Western blot assays revealing the protein levels of LDHA, HK2, P-YAP and YAP1 in tumour tissues from miR-103a-3p antagomir group and control group. f Representative images showing vascular distribution and density within tumour tissues from miR-103a-3p antagomir group and control group by H&E staining (scale, 50 μm). g Immunohistochemical staining showing the protein levels of HK2, PKM1, LDHA, KI-67, PCNA, and P-YAP in tumour tissues from miR-103a-3p antagomir group and control group (scale, 50 μm). *p < 0.05, **p < 0.01, ***p < 0.001