Fig. 5

YAP1/TEAD1 promotes glycolysis and angiogenesis in CRC by activating the transcription of HIF1A. a The acidity of the cell nutrient solution was detected in HCT116 cells transfected with vector, HIF1A, si-control, or si-HIF1A under hypoxia. b The expression correlation of HIF1A with the glycolytic gene HK2 or LDHA in CRC samples by TCGA. c Western blot and angiogenesis d assays showing the expression of VEGFA, LDHA and HK2 and angiogenesis in HCT116 cells transfected with vector, HIF1A, si-control, or si-HIF1A under hypoxia. e Correlation analysis between YAP1 and glycolysis-associated gene sets, as demonstrated by GSEA. ES, enrichment score; NES, normalized enrichment score. The transcript f-g and protein levels h of HIF1A were measured in HCT116 cells transfected with vector, YAP1, TEAD1, si-YAP1, si-TEAD1, or si-control by qRT-PCR and western blot. i The level of HIF1A was determined in HCT116 cells transfected with si-YAP+si-TEAD1 or si-control by qRT-PCR. j The HIF1A binding motif in HIF1A predicted from JASPAR matrix models. k Scheme of the potential binding sites of TEAD1 in the HIF1A upstream promoter region. l ChIP assays using anti-YAP1 and anti-TEAD1 antibodies were performed in HCT116 cells. *p < 0.05, **p < 0.01