Fig. 6

Case report: COVID-19 positive HNSCC patient. a Clinical history of COVID-19 positive HNSCC patient: at day 0 patient underwent surgical resection of the tumor that was formalin fixed and paraffin embedded. 2 days after surgery the patient developed pneumonia symptoms and nasopharyngeal swab SARS-CoV-2 test at day 6 was positive. SARS-CoV-2 test was also performed in FFPE tumoral tissue and resulted to be negative. b Clinical characteristics of COVID-19 positive HNSCC patient. c Well differentiated squamous cell carcinoma (G1) showing areas of keratinization with infiltration of muscular layer. d DNA sequencing results. e Immunostaining for p53 protein expression in COVID-19 positive HNSCC patient. f Real-time PCR curves from SARS-CoV-2 test run of RNA extracted from FFPE tumor tissue of HNSCC patient and from FFPE non-tumorous and tumor lung tissues of a lung cancer patient enrolled in 2011. The internal control was indicated by the red arrow. g qRT-PCR analysis of TMPRSS2 in 5 normal lung tissues (N) and 5 matched lung cancer tissues plus COVID-19 positive HNSCC tissue (T). h qRT-PCR analysis of miR-503-5p in 9 non-tumorous lung tissues (N) and 9 matched lung cancer tissues plus COVID-19 positive HNSCC tissue (T)