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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Tubulin Tyrosine Ligase Like 4 (TTLL4) overexpression in breast cancer cells is associated with brain metastasis and alters exosome biogenesis

Fig. 3

TTLL4 increases vesicle trafficking. a-c MDA-MB231 cells were transfected with vectors encoding for GFP-BDNF, mRFP-Rab5 or mRFP-Rab7 and vesicle trafficking (μm/sec) was analyzed by life cell imaging. Shown are mean values ± SD of three different experiments. d Control cells (including the Lego vector) were transfected with mRFP-Rab7 vectors, fixed after 24 h of incubation, stained by antibodies against polyglutamylation modification (GT335, green) and analyzed by confocal microscopy. Shown are representative images. Bar: 10 μm. White arrow: Rab7 vesicles colocalizing with polyglutamylated MTs, blue arrow: Rab7 vesicles non- colocalizing with polyglutamylated MTs. Yellow arrow: EVs

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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