Fig. 3

RP11-757G1.5 promotes CRC cell proliferation in vitro. a-b Effects of RP11-757G1.5 overexpression and knockdown on colony formation in CRC cells. c-d Effects of RP11-757G1.5 overexpression and downregulation on CRC cell proliferation were measured by a Edu assay. Scale bar, 25 μm. e-f Cell cycle analysis was conducted in HCT-116 and SW480 cells transfected with pcDNA3.1-757G1.5 and sh-757G1.5#1; sh-757G1.5#2, respectively. g-h Western blotting was performed to detect proliferation-associated antigen Cyclin D1 and PCNA expression in HCT-116, SW480 cells transfected with pcDNA3.1-757G1.5 or sh-757G1.5, respectively. *p < 0.05, **p < 0.01 by Student’s t-test. Data are representative of at least three independent experiments