Fig. 4

RP11-757G1.5 accelerates CRC cell migration and invasion in vitro. a-b Wound-healing assay was used to investigate the horizontal migration ability with RP11-757G1.5 overexpression or knockdown in CRC cells, and relative gap distance was calculated and plotted on a histogram. c-d The transwell assays were applied in both HCT-116 and SW480 cells to evaluate the CRC cell vertical migration and invasion abilities prior transfected with pcDNA3.1-757G1.5 or sh-757G1.5, respectively, and the number of cells was calculated and plotted on a histogram. Scale bar, 25 μm. e-f Immunoblotting analysis was performed to determine epithelial-mesenchymal transition marker E-cadherin and N-cadherin expression in HCT-116, SW480 cells transfected with pcDNA3.1-757G1.5 or sh-757G1.5, respectively. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test. Data are representative of at least three independent experiments