Fig. 2

WTX negatively regulates GC proliferation. a-d Cell proliferation analyses of the indicated cells. *P < 0.05, means±SD, n = 3. Two-tailed Student’s t-test. e, g Representative images from colony formation assays for the indicated cells. f, h Quantification of colony formation for the indicated cells. ***P < 0.001, *P < 0.05, means±SD, n = 3. Two-tailed Student’s t-test. i, l Images of gross GC orthotopic tumours formed by the indicated cell lines. j, m Representative images of GC orthotopic tumours with H&E staining. Scale bars, 50 μm. k, n Volume analyses for the GC orthotopic tumours. means ± SD