Fig. 6

MiR-20a-5p regulates GC progression through the WTX/PI3K/AKT/mTOR pathway. a The three-way Venn diagram shows the number of overlapping pathway components in the KEGG pathway enrichment analysis of AGS. W vs AGS.veh (n = 44, Fig. 3a), the GSE34715 dataset (n = 26, Figure S2c), and TCGA (n = 65, Figure S5c). b-c Western blot analyses of PI3K/AKT/mTOR pathway proteins in the indicated cells. GAPDH is shown as a loading control. d-f Colony formation analyses of the indicated cells. ***P < 0.001, **P < 0.01, means±SD, n = 3. Two-tailed Student’s t-test. g-h Cell proliferation analyses of the indicated cells. ***P < 0.001, means±SD, n = 3. One-way ANOVA. i ISH staining of miR-20a-5p and IHC staining of WTX, p-AKT, and p-mTOR in subcutaneous tumours formed by the indicated cells. Scale bars, 50 μm