Fig. 1
Cell number and cell death of human melanoma cells treated with increasing NAM concentrations. a, c: A375 and SK-MEL-28 cells were counted at 24 and 48 h following NAM treatment (1, 20, 50 mM). A375 (8 × 104) and SK-MEL-28 (1 × 105) cells were plated in p35 Petri dishes at time 0. Data are expressed as percentage of untreated cells. b, d: Cell death was quantified according to trypan blue exclusion assay and expressed as absolute numbers. All data are expressed as mean ± S.D. of three independent experiments. In each panel, statistical difference was achieved by one-way ANOVA test