Fig. 3
Cell cycle analysis of A375 cells treated with increasing doses of NAM. A375 cells were plated (8 × 104 cells/plate) in p35 Petri dishes at time 0 and were then starved for 18 h in serum-free medium. Cells were then treated with 0, 10, 20, and 50 mM NAM, for 24 h, in the presence of FBS 10%. Panels a, b, c, d report the mean ± S.D. of three independent experiments; 10.000 events were acquired. Panels e to h show a representative experiment. One-way ANOVA test in panels a, b and d computed a p < 0.05