Fig. 5

PI3K/AKT signaling pathway mediated SETDB1-induced CSF-1 induction. a Western blotting of indicated proteins in U87 cells transfected with SETDB1 overexpression with or without MK-2206 pretreatment. b Western blotting of indicated proteins in U251 cells transfected with SETDB1 overexpression with or without MK-2206 pretreatment. c Western blotting of indicated proteins in U87 cells transfected with SETDB1 overexpression with or without siRNA against AKT. d Western blotting of indicated proteins in U251 cells transfected with SETDB1 overexpression with or without siRNA against AKT. e Western blotting of indicated proteins in U87 cells transfected with SETDB1 overexpression with or without rapamycin pretreatment. f Western blotting of indicated proteins in U251 cells transfected with SETDB1 overexpression with or without rapamycin pretreatment. g Western blotting of indicated proteins in control and SETDB1 overexpression xenograft tumors. h Western blotting of indicated proteins in control and SETDB1 knockdown xenograft tumors