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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis

Fig. 2

miR-96 enhances cell migration, proliferation and invasion while repressing apoptosis of CRC cells by inhibiting AMPKα2. a: The expression of miR-96 in CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 determined by RT-qPCR. b: The protein expression of AMPKα2 in CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 measured by Western blot analysis. c: Viability of CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 assessed by CCK-8 method. d: Quantitative analysis for cell cycle distribution in CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 detected by flow cytometry. e: Quantitative analysis for apoptosis rate of CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 assessed by flow cytometry. f: The protein expression of proliferation-, cell cycle- and apoptosis-related genes in CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2, as measured by Western blot analysis; g: The migratory ability of CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 assessed by scratch test. h, The invasion ability of CRC cells co-transfected with miR-96 mimic and oe-NC/oe-AMPKα2 assessed by Transwell assay. * p < 0.05. Measurement data (mean ± standard deviation) between the two groups were compared by paired t test and those among multiple groups at different time points were compared by repeated measures ANOVA with Bonferroni post-hoc test. The cell experiment was independently repeated three times

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