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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis

Fig. 4

Demethylase FTO elevates the expression of MYC by blocking the m6A modification of MYC in CRC cells. a: The mRNA expression of MYC in clinical CRC tissues and non-tumor tissues determined by RT-qPCR (n = 60 for patients in each group). b: The mRNA expression of MYC in CRC cells and HIEC determined by RT-qPCR. c: The protein expression of FTO and MYC in CRC cells after transfection with sh-FTO measured by Western blot analysis. d: The m6A modification level of MYC in CRC cells assessed by Me-RIP assay. e: The binding relationship between FTO and MYC mRNA examined by PAR-CLIP assay; *p < 0.05. Measurement data (mean ± standard deviation) between the two groups were compared by paired t test and those among multiple groups were compared by one-way ANOVA with Tukey’s post hoc tests. Cell experiment was independently repeated for three times

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