Fig. 3

KDM5A potentiates PCa cell proliferation, invasion and migration while suppressing cell apoptosis by inhibiting miR-495. a, miR-495 expression in cancer and adjacent normal tissues of 78 patients detected by RT-qPCR. b, Correlation between miR-495 and KDM5A in cancer and adjacent normal tissues of 78 patients. c, miR-495 expression in RWPE-1, LNCaP, C42, DU145 and PC3 cells detected by RT-qPCR. d, The binding of KDM5A to the promoter of miR-495 confirmed by dual luciferase reporter assay. e, miR-495 expression in oe-KDM5A-transfected PC3 cells. f, The binding of KDM5A to the promoter of miR-495 verified by ChIP assay. g, Western blots of H3K4me3 in the antibody-transcription factor complex pulled down by KDM5A antibody. h, KDM5A mRNA expression in PC3 cells following varied transfection detected by RT-qPCR. i, Western blot analysis of KDM5A protein in PC3 cells following varied transfection. j, miR-495 expression in PC3 cells following varied transfection detected by RT-qPCR. k, PC3 cell proliferation following varied transfection measured by Colony formation assay. l, PC3 cell apoptosis following varied transfection measured by flow cytometry. m, PC3 cell invasion following varied transfection measured by Transwell assay. Scale bar = 50 μm. n, PC3 cell migration following varied transfection measured by Scratch test. * p < 0.05 vs. adjacent normal tissues, RWPE-1 cells, or cells transfected with oe-NC or oe-NC + mimic NC. # p < 0.05 vs. cells transfected with oe-KDM5A + mimic NC. ^ns p > 0.05 vs. cells transfected with oe-NC + miR-495 mimic or oe-KDM5A + mimic NC. Data in panel A were analyzed by paired t-test, in panel D-G by unpaired t-test and those in panel H-J were analyzed by one-way ANOVA with Tukey’s post hoc test