Fig. 1
The effects on cell proliferation and cell morphology after doxorubicin-multiple dose exposure. a The antiproliferative effects on TNBC cell lines, Hs578T and MDA-MB-231, measured using MTT assay using stepwise concentrations of doxorubicin (0.1, 1, 10, 50, 75, 100 μM) to determine the IC50 values at P0, P12 and P24; data are represented as Log (conc, μM) = log[concentration of doxorubicin, μM] (mean ± SD, n = 3). b The evaluation of morphological traits after doxorubicin exposure on TNBC cell lines (P12 and P24) though confocal microscopy. Actin filaments were staining using Phalloidin dye and nucleus was staining using DAPI dye. Scale represents 25 μm. Images were captured with 60x oil immersed objective (PLAPON60xOSC2,1.4 NA). c Alteration of nuclear cell morphology, evaluated by DAPI staining on blue channel (405 nm excitation). Magenta arrows represent the formation of the micronucleus, yellow arrows point multinucleate/polynuclear cells and blue arrows point cytoskeleton damage