Fig. 4

MYLK-AS1 up-regulates E2F7 expression by competitively binding miR-424-5p in HCC. a-b Binding ability of MYLK-AS1, miR-424-5p, and E2F7 to anti-Ago2 in Hep-G2 and MHCC-97H (anti-igG was used as control) by RIP assay. c HEK-293FT cells co-transfected with wide type (WT) or mutated (Mut) E2F7 3′-UTR reporter vector and miR-424-5p mimic by luciferase reporter assay. d Relative E2F7 mRNA expression in Hep-G2 cells after MYLK-AS1 knockdown or in MHCC-97H cells after MYLK-AS1 overexpression. e E2F7 mRNA expression in MHCC-97H cells after reducing the expression of MYLK-AS1 and/or inhibition of miR-424-5p by qRT-PCR. f E2F7 mRNA expression in MHCC-97H cells following the ectopic expression of miR-424-5p and/or pLVX-E2F7 expression vector lacking the 3′-UTR by qRT-PCR. Results are expressed as mean ± SD. n.s, not significant, *P < 0.05, **P < 0.01, and ***P < 0.001