Fig. 3

Overexpression MCL1 restores cell proliferation and decreases apoptosis induced by lycorine hydrochloride. a The expression of MCL1 in MKN-45 and SGC-7901 cells treated with different concentration LH (10, 20 and 40 μM) for 48 h and treated with 20 μM LH for different time (0, 12, 24 and 48 h). DMSO was used as control. Tubulin was used as internal reference. b The expression of MCL1 in 20 μM LH-treated cells overexpressing MCL1 or empty vector. c Growth curve of MKN-45 and SGC-7901 cells overexpressing MCL1 after treatment with 20 μM LH. DMSO and empty vector were used as control. d The expression of MCL1, cleaved-PARP and C-Caspase3 were checked in MKN-45 and SGC-7901 cells overexpressing with MCL1 after treatment with 20 μM LH for 48 h. DMSO and empty vector were used as control. Tubulin was used as internal reference. e The interaction of MCL1 and BIM; BIM and BAX were detected after treating with different concentration LH (10, 20 and 40 μM) for 48 h by immunoprecipitation. DMSO was used as control. All data were analyzed by unpaired Student’s t-tests and were showed as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001