Fig. 5
Lycorine hydrochloride induces apoptosis of BCL2-drug-resistant gastric cancer cell lines. a Screening of HA14–1 resistant cell lines (Detailed screening methods was described in the materials and methods). b Western blotting analysis of MCL1 expression in MKN-45-R and SGC-7901-R cell lines expressing shGFP, shMCL1#2, shMCL1#4. Trypan blue staining was used to analyze the apoptosis induced by DMSO or HA14–1 in MKN-45-R and SGC-7901-R cell lines expressing shGFP, shMCL1#1. c, d Apoptosis was analyzed in MKN-45-R, SGC-7901-R cells after treatment with 20 μM LH for 48 h by trypan blue and TUNEL staining. Apoptotic rate of MKN-45-R, SGC-7901-R cells in panel was quantified. e, f The expression of apoptotic protein, including C-Caspase 9, C-Caspase 3, PARP and C-PARP in BCL2-drug-resistant gastric cancer cells treated with LH at different concentrations and time gradients. DMSO was used as control. Tubulin was used as internal reference. All data were analyzed by unpaired Student’s t-tests and were showed as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001