Fig. 5

miR-144 down-regulation promotes proliferation, migration and invasion and inhibits apoptosis in RCC cells. a, Expression of miR-144 in RCC tissues and adjacent normal tissues measured using RT-qPCR (n = 77). b, Correlation analysis between circ_001287 and miR-144. c, Relative expression of miR-144 in 786-O cells after treatment with miR-144 inhibitor using RT-qPCR. The 786-O cells were transfected with miR-144 inhibitor, inhibitor NC, or si-circ_001287 + miR-144 inhibitor. d, Viability of 786-O cells measured using CCK-8 assay. e, Cell cycle distribution measured using flow cytometry. f, Image (× 200) of migration of 786-O cells measured using Transwell assay. g, Quantification analysis of migration ability of 786-O cells measured using Transwell assay. h, Image (× 200) of invasion of 786-O cells evaluated using Transwell assay. i, Quantification analysis of invasion ability of 786-O cells evaluated using Transwell assay. j, Apoptosis in 786-O cell assessed through flow cytometry. k, PCNA, KI67, MMP2, MMP9, Cyclin D1, Cyclin E, Caspase-3, and Caspase-9 protein expression in 786-O cells measured using Western blot analysis. ^*p < 0.05 vs. adjacent normal tissues or 786-O cells treated with inhibitor NC; # p < 0.05 vs. the 786-O cells treated with miR-144 inhibitor. The data were measurement data and described as mean ± standard deviation. Paired t-test was used for data comparison between the two groups. Data among groups were compared with one-way ANOVA along with Tukey’s post hoc test. The data at different time points among different groups was analyzed by the repeated-measures ANOVA and Bonferroni’s post hoc test. The cell experiment was repeated 3 times. miR-144, microRNA-144; RT-qPCR, reverse transcription quantitative polymerase chain reaction; circRNA, circular RNA; CCK-8, cell counting kit-8