Fig. 5

UPK1A-AS1 correlates with EZH2 targets. a. GSEA analysis plot indicated a significant correlation between UPK1A-AS1 and EZH2 targets (P < 0.05). b. Visualization of GSEA findings of the correlation between EZH2 and cell cycle gene signatures in HCC sample from TCGA dataset (P < 0.05). c. Heatmap of Gene Ontology (GO) enriched terms colored by P-values. d. Knockdown efficiency of si-EZH2 in SK-Hep-1 and MHCC-97H cells was measured by western blotting. e. Growth curve of HCC cells transfected with si-EZH2 (^***P < 0.001). f. RNA expression of the indicated genes after si-EZH2 was measured using qRT-PCR (^*P < 0.05, ^**P < 0.01, ^***P < 0.001). g. Overexpression of UPK1A-AS1 increased the expression of the indicated genes measured by qRT-PCR (^*P < 0.05, ^**P < 0.01, ^***P < 0.001)