Fig. 2

Overexpression of circMCTP2 facilitates the sensitivity of CDDP-resistant GC cells in vitro. a and b Reduction in CDDP-resistant GC cell viability by elevated expression of circMCTP2. c Impairment of the colony-forming ability of BGC823CDDP cells in response to cisplatin treatment after circMCTP2 overexpression. d CircMCTP2-mediated decrease in colony formation by SGC7901CDDP cells in the presence of cisplatin. e Assessment of DNA synthesis of BGC823CDDP and SGC7901CDDP cells transfected with NC and lentivirus-circMCTP2 in the presence of cisplatin. f and g Flow cytometric analysis of the apoptosis of CDDP-resistant GC cells treated with cisplatin. h Detection of apoptosis-related proteins by western blotting in BGC823CDDP and SGC7901CDDP cells. CDDP treatment: 12 μM for 48 h in BGC823CDDP cells and 6 μM for 48 h in SGC7901CDDP cells. (*p < 0.05, **p < 0.01. Data are expressed as the means ± SDs)