Fig. 8

Exosomal miR-146a-5p exhibits its functions by inhibiting EGFR/ERK pathway in PCa cells. a and b Migration and invasion of LNcaP cells transfected with lenti-miR-control, lenti-miR-146a-5p, lenti-miR-146a-5p mock, or lenti-miR-146a-5p EGFR-OE were evaluated by a Transwell assay (scale bars = 25 μm). c and d Migration and invasion of DU145 cells transfected with lenti-miR-control, lenti-miR-146a-5p, lenti-miR-146a-5p mock, or lenti-miR-146a-5p EGFR-OE were determined using a Transwell assay (scale bars = 25 μm). e After transfection with lenti-miR-control, lenti-miR-146a-5p, lenti-miR-146a-5p mock, or lenti-miR-146a-5p EGFR-OE, the protein level of E-cadherin, Vimentin, EGFR, ERK, p-ERK in PCa cells were evaluated using Western blot analysis. f Immunohistochemistry analysis showing the expression of p-ERK in lung metastasis among three groups (scale bars = 12.5 μm). g Representative Immunohistochemical staining for E-cadherin, Vimentin, EGFR and p-ERK in androgen deprived prostate tissues (Castration) and the control PCa tissues (NC) (n = 8). Data are shown as mean ± SD representing triplicate measurements. (Student’s t-test, * P < .05, **P < .01, ***P < .001, ****P < .0001)