Fig. 2

Effects of LAT1 inhibition in aortic ring assay and in Matrigel plug assay. a Whole-mount immunofluorescence of LAT1 and Claudin-5 in aortic rings. Dashed lines; edge of aortic rings. Asterisks; autofluorescence from aortic rings. b Aortic ring assay performed in the presence or the absence of 50 μM JPH203. Bar graph; quantification of endothelial sprouts. c Immunofluorescence of LAT1 and CD31 in Matrigel plug frozen section. d Fluorescent images of isolated Matrigel plugs implanted with or without 50 μM JPH203. Bar graph; quantification of FITC fluorescence. a, c LAT1 was stained with mLAT1 (c) antibody. Nuclei were stained with DAPI (blue, in merged image). b, n = 12; d, n = 16 for non-treated control (−), n = 13 for JPH203